선바이오(주)

PEG-hydrazide can pegylate carboxyl group quite selectively in presence of N,N'-dicyclohexylcarbodiimide (DCC), or in presence of a water soluble coupling agent such as N-(-3dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC).

The very low pKa (~3) of the hydrazide group allows carboxyl pegylation in acidic media in presence of EDC. The carboxyl groups of a protein which are readily activated with EDC at mild acidic pH, tend to react readily with PEG-hydrazide, whereas amino groups of the protein remain non-active.

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    Hydrazide derivatives of polyethylene glycol and their bioconjugates, 1997 American Chemical Society.